Alcoholic fatty liver is associated with insulin resistance (Zong et al., 2012). Indeed, insulin receptor (IR)-mediated signaling molecules PI3K/AKT and IRS-1 were decreased in response to ethanol feeding (Fig 1D, 1E). However, there were no differences in the decrease in these insulin signaling molecules between Cyp2a5−/− mice and Cyp2a5+/+ mice (Fig 1D, E), suggesting that the ethanol-induced insulin resistance may not explain the difference in development of alcoholic fatty liver between Cyp2a5−/− mice and Cyp2a5+/+ mice.
