For cell death assays, iNPCs and primary GTICs were treated with 5, 10 and 20 μM for the indicated chemotherapeutic compounds in duplicates in six-well-plates. Human glioma lines (U-87 MG and LN229) were treated with 1 mM for the indicated compounds. 24 h after treatment, cells were dissociated by Accutase (Innovative Cell Technologies, cat. no. AT-104) and Annexin V/PI staining conducted following the manufacturer's recommendations (88-8007-72 Annexin V APC Ebiosciences). PI staining served to exclude dead cells and only Annexin V cells present in the PI negative living cell population (indicative of early apoptosis) were considered for analysis. All stainings were done in biological triplicate with technical triplicates (n=9 total) per line and per condition.
