For flow cytometry analysis, cells were harvested using TrypLE (Invitrogen), washed once with PBS and further incubated with the corresponding antibodies in the presence of FACS blocking buffer (1 × PBS/10%FCS) for 1 h on ice in the absence of light. After incubation, cells were washed thrice with 1 ml of FACS blocking buffer and resuspended in a total volume of 200 μl before analysis using an LSRII instrument (Becton-Dickinson, Fullertone, CA). A minimum of 10,000 cells in the living population was analysed. Percentages are presented after subtracting isotype background and referring to the total living population analysed. Results are representative of at least two independent experiments with a minimum of two technical replicates per experiment (n⩾4) and per condition.
