SVZ cells were infected using a Notch Intracellular domain (Ad-NICD) or control (Ad-LacZ) construct Adenovirus (gift of Dr. Igor Prudovsky) by direct injection into the LV. Adenovirus production and titer determination were previously described37. P90 WT and CNP-hEGFR mice were injected with the Adenovirus stock (2μl; titer of 2–4 × 106 cfu/ml). Injections were performed stereotaxically at the following coordinates (anterioposterior relative to bregma, mediolateral, and dorsoventral from surface of the brain) for epl-SVZ (0, 1.8, 3.0mm). Brains were processed for histology at 2, 7, 14 and 28 days after infection, and sections were immunostained for NICD, GFAP, S100b, NG2 and BrdU antibodies.
