Chunk #39 — STAR Methods — Experimental Model and Subject Details — Cell Culture — Maintenance and Culture of Human Pluripotent Stem Cells (hPSCs)

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iPSC-Derived Human Microglia-like Cells to Study Neurological Diseases.

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iPSCs were maintained in 6-well plates (Corning) in feeder-free conditions using growth factor-reduced Matrigel (MTG, BD Bioscience) in complete TeSR-E8 medium (StemCell Technologies) in a humidified incubator (5% CO2, 37°C). iPSCs were fed fresh media daily and passaged every 7–8 days.