In total, data were collected from 40 subjects and both abdominal and gluteal adipose tissue, 13 of the 80 samples were measured in duplicates, resulting in data from 93 arrays available for further analysis in the replication study. 500 ng of total RNA from each sample were labelled and hybridized on Universal-12 Illumina BeadChip (Cat. No. # 11288011), according to the manufacturer's recommendations (Illumina microRNA expression profiling assay for BeadChips). BeadChips were scanned with the Illumina BeadArray scanner accordingly and images imported into GenomeStudio version 1.6.0 (illumina) to extract the raw data for further analysis.
