Chunk #35 — Materials and Methods — Study design and experimental material and methods — mRNA expression profiling

Source

MicroRNA expression in abdominal and gluteal adipose tissue is associated with mRNA expression levels and partly genetically driven.

Embedded

yes

Text

Total RNA was extracted with TRIreagent (SIGMA-ALDRICH) from fat biopsies. RNA expression was analyzed on the Affymetrix human GeneChip HGU133 Plus 2.0 array (Affymetrix) covering over 47,000 transcripts genome-wide. Labelled RNA was hybridized onto Affymetrix arrays, washed, stained and scanned for fluorescence intensity corresponding to gene expression level.