for 10 minutes prior to application of the kainate receptor agonist ATPA ((RS)-2-Amino-3-(3-hydroxy-5-tert-butylisoxazol-4-yl) propanoic acid; 5 μM) for 15 minutes; the slice was then washed for 40 minutes in drug-free aCSF. fEPSP recordings were acquired with an Axoclamp 2B amplifier (Axon Instruments, Foster City, CA) in the current-clamp mode and digitized with a Digidata 1200B (Axon Instruments). All values were expressed as mean percent control ± SEM. Data were subjected to a one way ANOVA or a two-way ANOVA (see text) with P<0.05 considered statistically significant.
