individuals compared to controls was located in the IL-1α locus (Figure 1B and Figure S1B), located 27 Kb upstream of IL-1α. The region is of interest because its methylation levels are significantly correlated with IL-1α levels (R = 0.52; FDR <0.14), and it contains many regulatory elements identified by ENCODE (Figure 1B bottom, highlighted in blue). These include a DNase hypersensitive site, H3K4Me1 and H3K27Ac enrichment peaks and several transcription factor-binding sites, including those of both activators and repressors. Transcription factors BATF, NFkB and PU.1 have the strongest binding signal in this region.
