BK channel clusters that can be activated by this RYR2 cluster, we obtained a STOC with an amplitude of 35 pA, a TTOD of 22 ms, and a τdecay of 31 ms. On average, simulated STOCs had an amplitude of 114 ± 15.3 pA, with a TTOD of 22.9 ± 3.2 ms and a τdecay of 38 ± 5.8 ms (n = 6 cells and 862 pairs of RYR2 and BK channels; Fig. 7 B). When held at 0 mV, these cells generated STOCs with an amplitude of 95 ± 14 pA, a TTOD of 29.4 ± 2.7 ms, and a τdecay of 34.9 ± 3.4 ms (n = 28 cells). Therefore, simulated STOCs exhibit similar amplitude and kinetics as experimental STOCs in these cells. This conclusion is further strengthened by comparing the effects of varying ICa(spark)s (Fig. S2) and voltage (see following section) on simulated and measured STOCs.
