reactions, 0.5μL FAM-labeled SEMA5A probe and 0.5μL VIC-labeled MAP2 probe were used per 10μL reaction. The amount of SEMA5A relative to MAP2 was determined for each case using the ΔΔCt method 43. Comparison of SEMA5A to TBP and GAPDH yielded similar results. Logistic regression was performed on autism status, adjusting for age at death, post-mortem interval, sex, and SEMA5A expression, with a 1-sided P-value reported for the association of lower SEMA5A expression with autism status.
