To determine an appropriate experimental threshold for genome-wide significance, permutation was performed on this dataset by gene-dropping, and genome-wide significance was estimated by taking the lowest P-value from each of 1000 permuted datasets and using the 50th as a threshold for P < 0.05 experiment-wide significance (P<2.5 × 10−7). To calculate an estimate of the effective number of tests (Teff), we used the following algorithm:
