of pGEX-4T1 (Pharmacia Biotech) to make pARID2-pGEX. The PLU-1 sequence was generated by RT–PCR from MCF-7 cells using oligonucleotides PLU-1 For (5′-TTCGCGGACCCCTTCGCTTTCA) and PLU-1 Rev short (5′-AATATTCATGGCCTCTGCTCTC). The reaction generated a 597 bp fragment extending from nucleotide 213 to 810 (according to accession number AJ132440.1), which was cloned into the pCR2.1-TOPO vector to create pPLU-1-TOPO. A PLU-1 containing BstXI restriction fragment was released from the vector, blunted with T4 DNA polymerase, and ligated with SmaI digested pGEX-4T1 to generate pPLU-1-GST. pPLU-1-GST generates a GST-fusion protein containing amino acids 42–241 of PLU-1 according to accession number CAB43532. An RBP2 sequence-containing PCR fragment was generated with primers RBP2-F-Xho (5′-AGACTCGAGTTCACAGATCCGCTCAGCTTTATC) and RBP2-R-Xho (5′-AGACTCGAGTTTAGGACACCTCCAGTCTCCTTT) from the plasmid template pCMV-HA-RBP2 (provided by Philip Branton), and cloned into the pCR2.1-TOPO vector to create pRBP2-TOPO. An XhoI restriction fragment from the RBP2-TOPO insert was blunted with Klenow polymerase and ligated with SmaI-digested pGEX-4T3 to create the plasmid pRBP2-pGEX. This construct produces a GST-fusion protein containing RBP2 amino acids 29–339 (accession number NP_005047). The jumonji fragment was amplified by PCR from a murine brain cDNA library in a vector backbone of pACT-2 (Clontech) that was kindly provided by Dr Premkumar Reddy (Fels Institute, Temple University School of Medicine,
