acids 29–339 (accession number NP_005047). The jumonji fragment was amplified by PCR from a murine brain cDNA library in a vector backbone of pACT-2 (Clontech) that was kindly provided by Dr Premkumar Reddy (Fels Institute, Temple University School of Medicine, Philadelphia, PA). jumonji-TOPO was generated by PCR using the primers jumonji For (5′-AGAGAATTCTGTGAAAATCGTTCTACCTCGCAA) and jumonji Rev (5′-AGACTCGAGATGACAGTCCTTCTCTTCCACTAA) to generate a 1030 bp fragment extending from nucleotide 1750 to 2780 according to accession number D31967. The PCR fragment was cloned into the pCR2.1-TOPO vector to create pjumonji-TOPO, excised from the vector with EcoRI and XhoI, and cloned into EcoRI/XhoI-digested pGEX-4T1 to create pjumonji-pGEX-4T1. This construct creates a GST-fusion protein containing amino acids 519–858 of jumonji (accession number NP_068678). This is the only case where a murine sequence was used in the subfamily constructs, but the mouse and human proteins are 92% identical across the coding span of the insert.
