KIAA1212, a potential binding partner of DISC1 identified from a previous yeast two-hybrid screen (Camargo et al., 2007), has been shown to directly interact with AKT and strengthen AKT activation in cell lines (Anai et al., 2005). We first characterized the expression of KIAA1212 in proliferating adult neural progenitors and their progeny in culture (Figure S2). Interestingly, very little KIAA1212 was detected in proliferating adult neural progenitors at both the mRNA and protein levels. Instead, there was a gradual increase of KIAA1212 expression during neural differentiation of adult neural progenitors. In situ hybridization confirmed high level of KIAA1212 expression in the dentate granule cell layer of the adult hippocampus (Figure S2). Thus, KIAA1212 appears to function during differentiation and maturation of adult neural progenitors, but not during the proliferation stage.
