Several groups have published changes in brain gene expression related to alcohol and we have compared their data with the present study in Table S4. The studies included are a comparison of differences in genetic predisposition to alcohol consumption in mice [13], individual differences in alcohol consumption in mice [10], consumption of alcohol in alcohol-preferring rats [6], human alcoholics [3], [11], gene expression changes in three mouse models of ethanol consumption [66] and gene expression changes in mice after chronic intermittent ethanol exposure [67]. Table S4 also contains a comparison of our data with LPS-induced gene expression changes in mouse astrocytes [68]. Several genes from our dopamine-centric pathway (Figure 4A) are found in multiple studies. For example, DRD2, EGR2, KCNA5 and PDE10A were all found in by Mulligan et al. [13] and, as noted above, PDE10A was one of the top genes in the Wolstenholme et al [10] study. FOS, a main hub gene in our pathway analysis, was also changed in the Bell et al. [6] data and two other genes in our pathway, PDYN and DRD1, were changed
