To quantify levels of DRD1, HOMER1 and DLG4 gene expression in putamen and cingulate brain punches, cDNA was synthesized (Quanta BioSciences, Gaithersburg, MD) and subjected to real-time PCR analysis using Taqman-based probes (Applied Biosystems); all primers and probes were products of Assays-On-Demand from Applied Biosystems (DRD1, Hs00377719_g1; HOMER1, Hs00188676_m1; DLG4, Hs00176354_m1). Real-time PCR and subsequent analysis were performed with a Roche LightCycler 480 Real-Time PCR system (Roche Applied Science, Indianapolis, IN). Quantification of target gene expression in all samples, performed in triplicate, was normalized to 18S rRNA (4319413E; Applied Biosystems) using the ΔΔCT method.31
