Tissue samples were sonicated in SDS extraction buffer. Extracts were aliquoted and kept at −80°C until usage. Protein concentration was determined using the BCA protein assay (Fisher, Rockford, IL). Rabbit polyclonal antibody against HOMER1b/c (Abcam, Cambridge, MA) was used. Membranes were washed three times for 15 min in PBST buffer and incubated with goat anti-rabbit secondary antibody (LI-COR, Lincoln, NE) for 1h at room temperature. MemCode membrane staining (Fisher) was used to control for total protein content. Membranes were developed with the LI-COR detection system and the ImageJ software (NIMH, MD) was used for densitometric analysis.
