The miRNA microarray also included a set of probes corresponding to unpublished miRNA sequences from Ambion. Seven of these miRNAs were differentially expressed in the frontal cortex of alcoholics (ambi_miR_12897, ambi_miR_9451,ambi_miR_8488,ambi_miR_9125,ambi_miR_7058, ambi_miR_8518, and ambi_miR_13196). The sequences corresponding to the probes on the microarrayfor each of these miRNAs were compared with the sequences of the known miRNAs registered in miRBase using the SSEARCH algorithm (http://www.ebi.ac.uk). Only one, ambi_miR_7058, showed homology to a known miRNA. This miRNA sequence exhibited a 100% match to the hsa-miR-423-5p sequence. The predicted targets for this miRNA were included in subsequent analyses. Thus, a total of 35 differentially expressed miRNAs were used for target predictions (Table 1).
