Up-regulation of microRNAs in brain of human alcoholics.

paper Cited Public

Authors: Lewohl, Joanne M; Nunez, Yury O; Dodd, Peter R; Tiwari, Gayatri R; Harris, R Adron; Mayfield, R Dayne
Year: 2011
Journal: Alcoholism, clinical and experimental research
PMID: 21651580
DOI: 10.1111/j.1530-0277.2011.01544.x
PMCID: PMC3170679

#	Section	Preview
0	Introduction	In the brain, the acute effects of alcohol are mediated by direct interaction with neurotransmitter…
1	Introduction	Gene and protein expression profiling techniques have been used to identify alcohol-induced changes…
2	Introduction	region of the brain that is particularly susceptible to the effects of long-term alcohol abuse and…
3	Introduction	MicroRNAs (miRNAs) are small, non-coding RNAs that are thought to act as post-transcriptional…
4	Introduction	Recent studies have revealed that miRNAs are highly abundant in brain and play important roles in a…
5	Introduction	Pietrzykowski et al (Pietrzykowski et al., 2008)showed that alcohol, acting via a specific miRNA,…
6	Introduction	In the present study, we used miRNA arrays to identify miRNAs with altered expression in the frontal…
7	Materials and Methods — Case selection	Tissues were received from the Queensland Brain Bank, School of Molecular & Microbial Sciences, The…
8	Materials and Methods — Case selection	A total of 27 cases including 14 alcoholic and 13 control cases were selected for analysis. The…
9	Materials and Methods — MiRNA extraction and hybridization	MiRNAs were extracted using the mirVana™ miRNA Isolation Kit (Ambion, Austin TX, USA) according to…
10	Materials and Methods — MiRNA extraction and hybridization	Microarray printing and hybridization were performed at the NIH-Duke University microarray facility.…
11	Materials and Methods — MiRNA microarray data analysis	MiRNA array data analysis. Data preprocessing included normexp background correction (Ritchie et…
12	Materials and Methods — Validation by real time RT-PCR analysis	Single-stranded cDNA was synthesized from total RNA using the TaqMan® MicroRNA Reverse…
13	Materials and Methods — MiRNA target prediction	Gene targets of the differentially expressed miRNAs were predicted using the Predicted Targets…
14	Materials and Methods — Integrative analysis of miRNA and mRNA microarray data	Hypergeometric tests for multiple experimental proportions extracted from the integrated dataset…
15	Materials and Methods — Integrative analysis of miRNA and mRNA microarray data	To verify the results from the hypergeometric tests on the experimental data, we generated 1000…
16	Materials and Methods — Gene list functional enrichment analysis	Lists of statistically significant miRNAs and mRNAs were subjected to functional enrichment analysis…
17	Results — MiRNA up-regulation in the frontal cortex of alcoholics	MiRNA microarrays were used to measure miRNA expression profiles in the frontalcortex of control and…
18	Results — MiRNA up-regulation in the frontal cortex of alcoholics	Approximately48 miRNAs were up-regulatedin the frontal cortex of alcoholics (p < 0.005, FDR < 15%)…
19	Results — MiRNA up-regulation in the frontal cortex of alcoholics	The miRNA microarray also included a set of probes corresponding to unpublished miRNA sequences from…

Name	Type
7900HT Fast Real-Time PCR System local	drug
ABI2 local	gene
Acute effects of alcohol local	phenotype
Agilent 2100 Bioanalyzer	drug
ALCAM local	gene
alcohol	phenotype
alcohol abuse	phenotype
alcohol dependence	phenotype
Alcoholic cases local	cohort
alcoholism	phenotype
alcohol-related cellular mechanisms local	phenotype
alcohol tolerance	phenotype
Alzheimer's disease	phenotype
Alzheimer’s disease	phenotype
ambi_miR_12897 local	gene
ambi_miR_13196 local	gene
ambi_miR_7058 local	gene
ambi_miR_8488 local	gene
ambi_miR_8518 local	gene
ambi_miR_9125 local	gene
ambi_miR_9451 local	gene
Ambion	drug
Animal models of alcoholism local	cohort
ANP32E local	gene
Applied Biosystems	drug
Australian Brain Bank Network local	cohort
Australian Brain Donor Programs NSW Tissue Resource Centre local	cohort
Axon GenePix 6.0 local	drug
BIRC6 local	gene
BK channel	gene
brain	anatomy
C11ORF9 local	gene
cancer	phenotype
CANX local	gene
CAPN3 local	gene
CCDC88A	gene
CDC42 local	gene
CDKN1B local	gene
chronic alcoholism	phenotype
CNP local	gene
cocaine	phenotype
cognitive decline	phenotype
Complicated alcohol-related disorders local	phenotype
control	cohort
control group	cohort
controls	cohort
core of 17 downregulated mRNAs local	gene
Cultured cells local	cohort
dementia	phenotype
Dendritic complexity	phenotype
Dendritic density local	phenotype
DICER1	gene
DIDO1 local	gene
downregulated mRNA (group of 131) local	gene
down-regulated mRNAs local	gene
DST local	gene
EDIL3 local	gene
ELOVL4 local	gene
Elovl5	gene
ELOVL7 local	gene
ethanol consumption	phenotype
experimental dataset local	cohort
FAR1 local	gene
FBXL3 local	gene
FERMT2 local	gene
frontal cortex	anatomy
GenExsoftware local	drug
heavy drinking	phenotype
hepatic encephalopathy	phenotype
hsa-let-7g local	drug
hsa-let-7g local	gene
hsa-miR-152 local	drug
hsa-miR-152 local	gene
hsa-miR-15b local	drug
hsa-miR-15b local	gene
hsa-miR-301a local	drug
hsa-miR-301a local	gene
hsa-miR-369-3p local	drug
hsa-miR-369-3p local	gene
hsa-miR-423-5p local	gene
hsa-miR-7 local	drug
hsa-miR-7 local	gene
human alcoholics	phenotype
LAMP2 local	gene
LASS2 local	gene
let-7 local	drug
let-7g local	gene
Liu et al., 2006 local	cohort
Liver cirrhosis	phenotype
LMO7 local	gene
microRNA (miRNA) local	drug
miR-144 local	drug
miR-144 local	gene
miR-146a local	drug
miR-152 local	gene
miR-15b local	gene
miR-18a local	drug
miR-18a local	gene
miR-194 local	drug
miR-203 local	drug
miR-203 local	gene
miR-301a local	gene
miR-339-5p local	drug
miR-339-5p local	gene
miR-34c-5p local	drug
miR-34c-5p local	gene
miR-380 local	drug
miR-380 local	gene
miR-519b-3p local	drug
miR-580 local	drug
miR-580 local	gene
miR-586 local	drug
miR-586 local	gene
miR-665 local	drug
miR-7 local	drug
miR-7 local	gene
miR-802 local	drug
miR-802 local	gene
miR-9	drug
mirBase 9.0 local	drug
miRNA	drug
miRNA-AI microarray local	drug
miRNA expression changes local	phenotype
miRNA Recognition Elements (MREs) local	drug
miRNAs	drug
miRNA samples local	drug
mirVana miRNA Isolation Kit local	drug
mirVana miRNA Labeling Kit local	drug
morphine	drug
mRNA	drug
multiple miRNAs local	gene
Neonatal rats	cohort
neuroadaptation	phenotype
neurodegenerative disease	phenotype
nicotine	drug
NIH-Duke University microarray facility local	cohort
OSBPL11 local	gene
over-targeted mRNA group local	gene
Pelizaeus-Merzbacher disease	phenotype
PIK3C2B local	gene
PLCL1	gene
PLP1	gene
prefrontal cortex	anatomy
Queensland Brain Bank local	cohort
randomized control group local	cohort
RDX local	gene
RIPK2 local	gene
RNU44 local	gene
RNU6B local	gene
RYBP local	gene
SCARB2 local	gene
SCD	gene
SGBC local	gene
SGK1 local	gene
SGMS1 local	gene
SMARCA5	gene
SNORD44 local	gene
SPAST local	gene
Spastic paraplegia 2 local	phenotype
SPG20 local	gene
SPOCK1 local	gene
SPTLC2 local	gene
study cohort	cohort
TaqMan® MicroRNA Assays local	drug
TaqMan® MicroRNA Reverse Transcription Kit local	drug
TEX2 local	gene
TGFB2	gene
TIMP2 local	gene
TKT local	gene
TNKS	gene
TP53INP1 local	gene
U6BsnRNA local	gene
UFSP2 local	gene
ULK2 local	gene
up-regulated miRNAs local	drug
up-regulated miRNAs local	gene
upregulated miRNAs (group of 35) local	drug
USP31 local	gene
Wernicke Korsakoff syndrome local	phenotype
Wernicke-Korsakoff syndrome	phenotype

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In this knowledge base

Title	Year	PMID
Alcohol reverses the effects of KCNJ6 (GIRK2) noncoding variants on excitability of human glutamatergic neurons.	2023	36207584
Exploration of alcohol use disorder-associated brain miRNA-mRNA regulatory networks.	2021	34601489
Gene expression profiling in the human alcoholic brain.	2017	28254370
Gene expression in the addicted brain.	2014	25172478
Neuroimmune signaling: a key component of alcohol abuse.	2013	23434064
Gene Expression Under the Influence: Transcriptional Profiling of Ethanol in the Brain.	2012	24078902

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Silencing synaptic MicroRNA-411 reduces voluntary alcohol consumption in mice.	Most D et al.	—	2019	→
A Whole Methylome Study of Ethanol Exposure in Brain and Blood: An Exploration of the Utility of Peripheral Blood as Proxy Tissue for Brain in Alcohol Methylation Studies.	Clark SL et al.	—	2018	→
Circulating MicroRNA Expression Levels Associated With Internet Gaming Disorder.	Lee M et al.	—	2018	→
Deep sequencing and miRNA profiles in alcohol-induced neuroinflammation and the TLR4 response in mice cerebral cortex.	Ureña-Peralta JR et al.	—	2018	→
FMSM: a novel computational model for predicting potential miRNA biomarkers for various human diseases.	Sun Y et al.	—	2018	→
Gender-Specific Effects of Selection for Drinking in the Dark on the Network Roles of Coding and Noncoding RNAs.	Iancu OD et al.	—	2018	→
Innate Immune Signaling and Alcohol Use Disorders.	Coleman LG et al.	—	2018	→
Long-term ethanol exposure: Temporal pattern of microRNA expression and associated mRNA gene networks in mouse brain.	Osterndorff-Kahanek EA et al.	—	2018	→
MicroRNAs as Immunotherapy Targets for Treating Gastroenterological Cancers.	Yang Y et al.	—	2018	→
miRNA-regulated transcription associated with mouse strains predisposed to hypnotic effects of ethanol.	Vestal B et al.	—	2018	→
Molecular Neuropathology of Astrocytes and Oligodendrocytes in Alcohol Use Disorders.	Miguel-Hidalgo JJ	—	2018	→
Potential Role of Extracellular Vesicles in the Pathophysiology of Drug Addiction.	Rao PSS et al.	—	2018	→
Predictive modeling of miRNA-mediated predisposition to alcohol-related phenotypes in mouse.	Rudra P et al.	—	2018	→
The miR206-JunD Circuit Mediates the Neurotoxic Effect of Methylmercury in Cortical Neurons.	Guida N et al.	—	2018	→
Alcohol effects on the epigenome in the germline: Role in the inheritance of alcohol-related pathology.	Chastain LG et al.	—	2017	→
A novel relationship for schizophrenia, bipolar and major depressive disorder Part 5: a hint from chromosome 5 high density association screen.	Chen X et al.	—	2017	→
Association of the PLCB1 gene with drug dependence.	Cabana-Domínguez J et al.	—	2017	→
Decoding the ubiquitous role of microRNAs in neurogenesis.	Nampoothiri SS et al.	—	2017	→
Determining causal miRNAs and their signaling cascade in diseases using an influence diffusion model.	Nalluri JJ et al.	—	2017	→
Emerging roles for ncRNAs in alcohol use disorders.	Mayfield RD	—	2017	→
Epigenetic mediators and consequences of excessive alcohol consumption.	Mahnke AH et al.	—	2017	→
Evidence linking microRNA suppression of essential prosurvival genes with hippocampal cell death after traumatic brain injury.	Boone DK et al.	—	2017	→
Exacerbation of Mild Hypoxia on Acute Radiation Syndrome and Subsequent Mortality.	Kiang JG	—	2017	→
Gene expression profiling in the human alcoholic brain.	Warden AS et al.	—	2017	→
Increased expression of microRNA-378a-5p in acute ethanol exposure of rat cardiomyocytes.	Wang Z et al.	—	2017	→
Microglial-derived miRNA let-7 and HMGB1 contribute to ethanol-induced neurotoxicity via TLR7.	Coleman LG et al.	—	2017	→
MicroRNA expression signature of methamphetamine use and addiction in the rat nucleus accumbens.	Sim MS et al.	—	2017	→
Pharmacoepigenomics of opiates and methadone maintenance treatment: current data and perspectives.	Marie-Claire C et al.	—	2017	→
Reprogramming of mPFC transcriptome and function in alcohol dependence.	Heilig M et al.	—	2017	→
The role of neuroimmune signaling in alcoholism.	Crews FT et al.	—	2017	→
Toll-like receptor signaling and stages of addiction.	Crews FT et al.	—	2017	→
Alcoholic liver disease and hepatitis C virus infection.	Novo-Veleiro I et al.	—	2016	→
A Single Nucleotide Polymorphism in the RASGRF2 Gene Is Associated with Alcoholic Liver Cirrhosis in Men.	Novo-Veleiro I et al.	—	2016	→
Decreased Expression of Plasma MicroRNA in Patients with Methamphetamine (MA) Use Disorder.	Zhao Y et al.	—	2016	→
Effects of addictive drugs on adult neural stem/progenitor cells.	Xu C et al.	—	2016	→
Environmental control of microRNAs in the nervous system: Implications in plasticity and behavior.	Codocedo JF et al.	—	2016	→
Expression of 14-3-3 transcript isoforms in response to ethanol exposure and their regulation by miRNAs.	Mathew DE et al.	—	2016	→
Impact of the Innate Immune Response in the Actions of Ethanol on the Central Nervous System.	Montesinos J et al.	—	2016	→
MicroRNA expression is altered in lateral septum across reproductive stages.	Saul MC et al.	—	2016	→
MicroRNAs and Drinking: Association between the Pre-miR-27a rs895819 Polymorphism and Alcohol Consumption in a Mediterranean Population.	Barragán R et al.	—	2016	→
MicroRNAs Are Involved in the Development of Morphine-Induced Analgesic Tolerance and Regulate Functionally Relevant Changes in Serpini1.	Tapocik JD et al.	—	2016	→
MicroRNAs as regulators of drug abuse and immunity.	Zhang K et al.	—	2016	→
Molecular mechanisms underlying alcohol-drinking behaviours.	Ron D et al.	—	2016	→
Plasma miRNA Profiles in Pregnant Women Predict Infant Outcomes following Prenatal Alcohol Exposure.	Balaraman S et al.	—	2016	→
Synaptic microRNAs Coordinately Regulate Synaptic mRNAs: Perturbation by Chronic Alcohol Consumption.	Most D et al.	—	2016	→
The Potential Role of Amygdaloid MicroRNA-494 in Alcohol-Induced Anxiolysis.	Teppen TL et al.	—	2016	→
Turing Revisited: Decoding the microRNA Messages in Brain Extracellular Vesicles for Early Detection of Neurodevelopmental Disorders.	Gillet V et al.	—	2016	→
Aberrant histone acetylation promotes mitochondrial respiratory suppression in the brain of alcoholic rats.	Jung ME et al.	—	2015	→
Alcohol Withdrawal and Cerebellar Mitochondria.	Jung ME	—	2015	→
Alterations in serum microRNA in humans with alcohol use disorders impact cell proliferation and cell death pathways and predict structural and functional changes in brain.	Ignacio C et al.	—	2015	→
Applying the new genomics to alcohol dependence.	Farris SP et al.	—	2015	→
Association of microRNA biogenesis pathway gene variants and alcohol dependence risk.	Gedik H et al.	—	2015	→
Corticostriatal microRNAs in addiction.	Heyer MP et al.	—	2015	→
Downregulation of Gabra4 expression during alcohol withdrawal is mediated by specific microRNAs in cultured mouse cortical neurons.	Bekdash RA et al.	—	2015	→
Integrating mRNA and miRNA Weighted Gene Co-Expression Networks with eQTLs in the Nucleus Accumbens of Subjects with Alcohol Dependence.	Mamdani M et al.	—	2015	→
MicroRNA-30a-5p in the prefrontal cortex controls the transition from moderate to excessive alcohol consumption.	Darcq E et al.	—	2015	→
Revisiting Chaos Theorem to Understand the Nature of miRNAs in Response to Drugs of Abuse.	Taki FA et al.	—	2015	→
Role of microRNAs in Alcohol-Induced Multi-Organ Injury.	Natarajan SK et al.	—	2015	→
The synaptoneurosome transcriptome: a model for profiling the emolecular effects of alcohol.	Most D et al.	—	2015	→
A genetic variant in the microRNA-146a gene is associated with susceptibility to alcohol use disorders.	Novo-Veleiro I et al.	—	2014	→
Converging actions of alcohol on liver and brain immune signaling.	Szabo G et al.	—	2014	→
Effects of Acute Prenatal Exposure to Ethanol on microRNA Expression are Ameliorated by Social Enrichment.	Ignacio C et al.	—	2014	→
Gene expression in the addicted brain.	Zhou Z et al.	—	2014	→
microRNA-206 in rat medial prefrontal cortex regulates BDNF expression and alcohol drinking.	Tapocik JD et al.	—	2014	→
MicroRNAs and ethanol toxicity.	Miranda RC	—	2014	→
Molecular basis of alcoholism.	Most D et al.	—	2014	→
Neuroimmune pathways in alcohol consumption: evidence from behavioral and genetic studies in rodents and humans.	Robinson G et al.	—	2014	→
Neuropathology of alcoholism.	Sutherland GT et al.	—	2014	→
Proteomic approaches and identification of novel therapeutic targets for alcoholism.	Gorini G et al.	—	2014	→
Stress-related alcohol consumption in heavy drinkers correlates with expression of miR-10a, miR-21, and components of the TAR-RNA-binding protein-associated complex.	Beech RD et al.	—	2014	→
Synaptic adaptations by alcohol and drugs of abuse: changes in microRNA expression and mRNA regulation.	Most D et al.	—	2014	→
Using autopsy brain tissue to study alcohol-related brain damage in the genomic age.	Sutherland GT et al.	—	2014	→
When less is more--microRNAs and psychiatric disorders.	Kolshus E et al.	—	2014	→
Coordinated dysregulation of mRNAs and microRNAs in the rat medial prefrontal cortex following a history of alcohol dependence.	Tapocik JD et al.	—	2013	→
Differential Effects of Chronic and Chronic-Intermittent Ethanol Treatment and Its Withdrawal on the Expression of miRNAs.	van Steenwyk G et al.	—	2013	→
Differential Expression of miR-130a in Postmortem Prefrontal Cortex of Subjects with Alcohol Use Disorders.	Wang F et al.	—	2013	→
Dysregulation of microRNA expression and function contributes to the etiology of fetal alcohol spectrum disorders.	Balaraman S et al.	—	2013	→
Epigenetic control of gene expression in the alcoholic brain.	Ponomarev I	—	2013	→
Integration of miRNA and protein profiling reveals coordinated neuroadaptations in the alcohol-dependent mouse brain.	Gorini G et al.	—	2013	→
Neurobiological signatures of alcohol dependence revealed by protein profiling.	Gorini G et al.	—	2013	→
Neuroimmune signaling: a key component of alcohol abuse.	Mayfield J et al.	—	2013	→
Over-expression of the miRNA cluster at chromosome 14q32 in the alcoholic brain correlates with suppression of predicted target mRNA required for oligodendrocyte proliferation.	Manzardo AM et al.	—	2013	→
Positively correlated miRNA-mRNA regulatory networks in mouse frontal cortex during early stages of alcohol dependence.	Nunez YO et al.	—	2013	→
The role of α-synuclein in the pathophysiology of alcoholism.	Janeczek P et al.	—	2013	→
Bioinformatic Resources of microRNA Sequences, Gene Targets, and Genetic Variation.	Mu W et al.	—	2012	→
Chronic intermittent ethanol exposure and its removal induce a different miRNA expression pattern in primary cortical neuronal cultures.	Guo Y et al.	—	2012	→
Gene Expression Under the Influence: Transcriptional Profiling of Ethanol in the Brain.	Contet C	—	2012	→
Genetic variation in hippocampal microRNA expression differences in C57BL/6 J X DBA/2 J (BXD) recombinant inbred mouse strains.	Parsons MJ et al.	—	2012	→
Global Approaches to the Role of miRNAs in Drug-Induced Changes in Gene Expression.	Eipper-Mains JE et al.	—	2012	→
MicroRNAs in Neurotoxicity.	Kaur P et al.	—	2012	→
The Emerging Role of Non-Coding RNAs in Drug Addiction.	Sartor GC et al.	—	2012	→
Understanding Alcoholism Through microRNA Signatures in Brains of Human Alcoholics.	Nunez YO et al.	—	2012	→