Chunk #9 — Materials and Methods — MiRNA extraction and hybridization

Source

Up-regulation of microRNAs in brain of human alcoholics.

Embedded

yes

Text

MiRNAs were extracted using the mirVana™ miRNA Isolation Kit (Ambion, Austin TX, USA) according to the manufacturer’s instructions. Yield and quality of the miRNA-enriched fraction was determined using the Agilent 2100 Bioanalyzer (Agilent, Palo Alto, CA, USA).