Gene targets of the differentially expressed miRNAs were predicted using the Predicted Targets component of miRecords ((Xiao et al., 2009), http://miRecords.biolead.org), which integrates the predicted targets produced by 11 established miRNA target prediction programs (DIANA-microT, Micro Inspector, miRanda, MirTarget2, miTarget, NBmiRTar, PicTar, PITA, RNA22, RNAhybrid, and TargetScan/TargertScanS). Predictions were filtered to only consider those targets predicted by at least 4 out of the 11 prediction algorithms.
