To determine the cellular source and relative expression of GSDMB and GSDMA in human lung we performed real-time PCR, and Western blots on primary normal airway epithelial cells and immunohistochemistry on formalin-fixed normal lung tissue (see Text S1 for details). Figure 3 shows abundant mRNA and protein expression of GSDMA but little GSDMB from primary human lung epithelial cells (Figure 3a and 3b respectively). Figure 3c shows that GSDMA is expressed in both apical and basal airway epithelial cells in the conducting airways.
