The Mantel correlations, similar to the Pearson correlations for coexpression, were used to assess DW. The annotations for the DW genes differed significantly from the coexpression annotations (see above). Enrichment was detected for Golgi vesicle-mediated transport, the nuclear pore and Ras GTPase binding. The latter annotation is consistent with several reports, although not from the perspective of splicing, that preference consumption is associated generally with intracellular signaling and specifically with Ras/MAPK pathways (Metten et al. 2014; Mulligan et al. 2006). The number of Ras GTPase affected genes was large (N = 65) and included several different gene families, e.g. Rab, Rap and Srga. From the network perspective, most of these Ras GTPase genes were ‘leaf’ nodes, i.e. showing moderate to low intramodular connectivity. However, there were five hub nodes, all of which were significantly affected by selection: superoxide dismutase (Sod1), TBC1 domain family member 9 (Tbc1d9), nucleoporin 50 [Nup50, cytoplasmic FMR1-interacting protein 1 (Cyfip1) and N-Myc-downstream regulated 1 (Ndrg1)]. The latter four are hub nodes in the low line and Sod1 is a hub in the high line. To our
