These data demonstrate that with neuronal differentiation, cells withdraw from cell cycle, express different sets of signaling molecules than iPSC and express largely overlapping sets of neuronal lineage markers. Enrichment of dorsal telencephalic transcripts, such as PAX6 and EMX2, and the cortical determinate LHX2 (ref.48) in control and expression of ventral determinates, such as PAX2 and NKX2-1 (basal forebrain progenitors49), involved in formation of the medial ganglionic eminence and GABAergic interneuron differentiation50 in BP neurons suggest that neuronal identity is altered, as mutual inhibition between PAX6 and NKX2-1 determines dorsal/ventral boundaries in the telencephalon.51
