Day (2): Cells were quickly and gently collected in to 50 ml conical using 10 ml Stripette® and centrifuged. Supernatant was aspirated to waste and media was changed to pre-equilibrated (at 5%O2, 5%CO2, 37°C for 1 hour) base media supplemented with FGF2 (50 ng/ml) and VEGF (50 ng/ml) and placed back in hypoxia incubator.
