Chunk #45 — STAR Methods — Experimental Model and Subject Details — Cell Culture — Differentiation of iPSCs to Hematopoietic Progenitor Cells (iHPCs)
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Day (4): Media was changed to 2 ml per well with basal media containing FGF2 (50 ng/ml), VEGF (50 ng/ml), TPO (50 ng/ml), SCF (10 ng/ml), IL-6 (50 ng/ml), and IL-3 (10 ng/ml) and placed at normoxia incubator.