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Chunk #18 — Materials and Methods — Genotyping and Ancestry Informative markers (AIM)

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Independent and Interactive Effects of OPRM1 and DAT1 Polymorphisms on Alcohol Consumption and Subjective Responses in Social Drinkers.
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OPRM1 A118G SNP genotypes in our sample included A/A (n=94), A/G (n=31) and G/G (n=2). In these same subjects, DAT1 VNTR included 10/10 (n=63), 10/9 (n=50), 10/<9 (n=3), 9/9 (n=9) and <9/<9 (n=2). The frequency of the OPRM1 A allele was 219 (86.2%), and the G allele frequency was 35 (13.8%). DAT1 A10 frequency was 179 (70.5%) and DAT1 A9 frequency was 75 (29.5%). Hardy–Weinberg tests indicated OPRM1 and DAT1 genotype frequencies were in equilibrium. The expected Hardy-Weinberg frequencies are 94.4, 30.2, and 2.4 for OPRM1. The expected Hardy-Weinberg frequencies for DAT1 are 63.1, 52.9, and 11.1, respectively. The p-values were 0.759 for OPRM1 genotypes and 0.975 for DAT1 genotypes.