Chunk #7 — Methods — PAXgene RNA Extraction

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Complex nature of SNP genotype effects on gene expression in primary human leucocytes.

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2.5 ml of peripheral blood was collected into a PAXgene tube (Becton Dickinson, UK, 762165). PAXgene vials were chosen to prevent density gradient centrifugation, immortalization or in vitro cell culture artifacts changing mRNA profiles.