The HEK-293 cell line was purchased from American Type Culture Collection. Advanced DMEM, T4 DNA ligase, HindIII, XhoI, and SstI, Accuprime Pfx DNA polymerase, SuperScript III reverse transcriptase reagents, OneShot Top 10 chemically competent cells, zeocin, geneticin, Lipofectamine 2000, and Ni-NTA agarose were purchased from Invitrogen. Ponasterone A (PonA) was purchased from A.G. Scientific. Dexamethasone (Dex) was purchased from EMD Chemicals. RNeasy Mini Kit and EndoFree Maxi-and Mini-prep Kits were obtained from Qiagen. Lightcycler 480 SYBR Green I master mix was purchased from Roche Diagnostics. Biolase DNA polymerase, dNTPs, magnesium chloride, and NH4 reaction buffer were purchased from Bioline. SYBR Green I was purchased from Cambrex. Human genomic DNA, the Dual Luciferase Reporter Assay System was purchased from Promega. NE-PER nuclear extraction reagents, Biotin 3′ end DNA labeling kit, and Lightshift chemiluminescent EMSA Kit were purchased from Pierce.
