pVgEcR (Invitrogen) encodes the fusion transcription factor used to generate ecdysone-inducible cells. pRL-RSV and pGL4.21 (Promega) were used in the luciferase reporter assays; pRL-RSV constitutively expresses Renilla reniformis luciferase, and SEPP1 promoter fragments were cloned into the pGL4.2.1 plasmid that contains firefly luciferase. The pLTRluc glucocorticoid reporter plasmid and pDsRed-hGR glucocorticoid receptor expression plasmid were gifts from Dr. Carol Lim (University of Utah).
