Chunk #6 — Materials & Methods — Cell Culture

Source

Selenoprotein P regulation by the glucocorticoid receptor.

Embedded

yes

Text

Human embryonic kidney line HEK-293, as well as all subsequently engineered cells, were cultured in Advanced DMEM medium containing 2% fetal bovine serum and 2mM L-glutamine. Cells were maintained at 37°C in a humidified incubator with 5% CO2.