HEK-293 were transfected with pVgEcR and selected for Zeocin resistance to generate stable expression of the VgEcR gene product and are referred to as 293-EcR. 293-EcR cells that conditionally express 15-LOX-1 and ΔIle662 15-LOX-1 were previously described (Yu et al. 2004; Cordray et al. 2007). Conditional expression of β-galactosidase in the 293-EcR was achieved using similar methods.
