An expression vector, pDsRed-hGR, that constitutively expresses a DsRed2-labeled, functional human GR was generously provided by Dr. Carol Lim, University of Utah. The 293-EcR cells were stably transfected with this expression vector and selected for neomycin resistance in order to study the effects of GR signaling in HEK-293 cells. These cells are referred to as EcR-GR.
