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Chunk #13 — Materials and methods — Dependence induction by alcohol vapor chambers

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Permanent impairment of birth and survival of cortical and hippocampal proliferating cells following excessive drinking during alcohol dependence.
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rate that can be regulated. The flask is placed on a heater in which the drops of alcohol hitting the bottom of the flask are vaporized. Air flow controlled by a pressure gauge is delivered to the flask and carries the alcohol vapors to the vapor chamber that contains the animal cages. The flow rate was set to deliver vapors that result in blood alcohol levels between 125-250 mg% (g/dL) or 27.2-54.4 mM (Gilpin et al., 2008a).