In light of the cell-type-specific RNA-seq and exposure conditions assayed (Figure 1A), we expected to see prominent expression of genes previously associated with MB function and or previously implicated ethanol-related behaviors. Using the “Vocabularies” search function on FlyBase (flybase.org), a list of genes was acquired representing “adult mushroom body” (FBbt:00003684) and ethanol-associated genes termed “behavioral response to ethanol” (FBbt:0048149). As expected, many of the MB-associated genes showed robust mean expression across our treatment libraries (Figure 1A, Figure S3A). Interestingly, many ethanol-associated genes were also detectable (Figure S3B).
