The BDGP6 Dm6 annotation of the fly transcriptome is comprised of 17560 genes, with 34741 corresponding transcript isoforms, where 10010 genes are uniquely represented by a single transcript isoform. According to the current information (flybase.org), the Dm6 contains roughly 85% protein-coding isoforms, 2% noncoding RNAs, 1.5% microRNAs, 0.9% transfer RNAs, 0.8% small nucleolar RNAs, and 9.7% other/nonannotated biotype sequences. When pooling all expression counts across treatments, our data were comprised of 92% protein-coding genes/isoforms, 0.9% noncoding RNAs, 0.06% microRNAs, 0.06% transfer RNAs, 0.05% small nucleolar RNAs, and 6.3% other/nonannotated sequences (Figure S1E). The characterization of our RNA-seq libraries shows that adult MB neurons are actively transcribing detectable protein-coding genes to allow for interesting differential expression analyses.
