Immunohistochemical images were captured using an Olympus BX51TRF microscope and ProgRes® CapturePro 2.5 digital camera system (Jenoptik, Germany). The Bioquant Nova Advanced Image Analysis System™ was used to assess histological area and neuron density (immuno-positive neurons/mm2) using a cell counting technique described previously (Coleman et al., 2009; Crews et al., 2004). We have shown previously that this method results in nearly identical percent changes between treatment and control groups as other unbiased stereological techniques (Crews et al., 2004). In order to confirm MRI volume findings, area measurements of the basal forebrain were taken using Bioquant on sections labeled for parvalbumin. Parvalbumin interneurons filled the nuclei of the basal forebrain, making landmarks and boundaries more easily identifiable. Up to six sections in three different regions, defined by distance from bregma, were measured. Anatomical landmarks were identified using the mouse atlas (Franklin and Paxinos, 2001) in the same regions as those analyzed by MRI. The landmarks for each bregma and the corresponding nuclei were as follows: +1.34 to +1.1-concentric horns of the forceps minor of the corpus callosum to the genu of
