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Chunk #21 — Methods — Immunohistochemistry and Image Analysis

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Adolescent binge drinking alters adult brain neurotransmitter gene expression, behavior, brain regional volumes, and neurochemistry in mice.
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and Paxinos, 2001) in the same regions as those analyzed by MRI. The landmarks for each bregma and the corresponding nuclei were as follows: +1.34 to +1.1-concentric horns of the forceps minor of the corpus callosum to the genu of the corpus callosum including the medial septum, vertical limb of diagonal band, and ventral pallidum; +0.86 to +0.14-narrowing of the corpus callosum and appearance of the lateral septum to the decussation of the anterior commissure which included the lateral septal regions, medial septum, ventral pallidum, substantia innominata, horizontal diagonal band, magnocellular preoptic, and lateral preoptic areas; +0.02 to −0.22-separation of the anterior commissure decussation to the disappearance of the anterior commissure posterior, using the posterior wings of the anterior commissure and the internal capsule as landmarks in conjunction with parvalbumin staining to triangulate the basal forebrain nuclei which included the ventral pallidum, substantia innominata, horizontal diagonal band, bed nucleus of striatum, magnocellular preoptic nucleus, anterior amygdaloid area dorsal, and the interstitial nucleus of the posterior limb of the anterior commissure.