We focus here instead on “multiple pool” strategies. With careful attention to a large number of small details, these approaches can provide accurate allele typing. Multiple pool approaches provide estimates of a) differences between disease vs control samples, b) variability within disease samples and c) variability within control samples. Assessment of the differences between disease and control in the context of assessments of the variability within disease samples and within control samples allows us to use standard statistical approaches to assess the significance of the results.
