4D+28 by the phenotype percentages shown in Figure 5a. “Calculated Neurogenesis” is shown in Figure 6 and specifically, that the fewer BrdU+ cells in the ethanol group (with a similar phenotype percentage) results in 53% fewer (p<0.01) new neurons in the ethanol group. There were no statistical effects of alcohol on the number of new glia or the number of “other” new cells not labeled by either NeuN or GFAP. Therefore, alcohol exposure in a model of an AUD results in decreased neurogenesis a month after exposure.
