The number of remaining BrdU+ cells was then quantified in an adjacent series of tissue stained with DAB colorization due to greater stability of the labeling method. After 28 days, BrdU+ cells were larger and rounder in appearance than the cells labeled and viewed at the 4D proliferation timepoint (see Figures 5 and 6). The cells were dispersed individually throughout the subgranular zone and the inner portion of the granule cell layer of the dentate gyrus. As shown in Figure 6, a 42.7% decrease (p<0.05) was observed in the number of BrdU+ cells in the dorsal dentate gyrus of the ethanol group (n=4) when compared to the controls (n=5). This data suggests that after binge alcohol exposure, newborn cell survival is decreased. The effects of alcohol on neurogenesis was then estimated by multiplying the number of remaining BrdU+ cells at 4D+28 by the phenotype percentages shown in Figure 5a. “Calculated Neurogenesis” is shown in Figure 6 and specifically, that the fewer BrdU+ cells in the ethanol group (with a similar phenotype percentage) results in 53% fewer (p<0.01) new neurons in
