In EA, rs1229984 was associated with Desire to cut drinking (p=1.21E-11). Two novel regions were GWS for two individual DSM4 criteria: rs188227250 on chromosome 8 for Drinking more than intended (p=6.72E-09); rs1912461 on chromosome 15 for Time spent drinking (p=1.77E-08). For the trans-ancestral (EA+AA) analysis, rs1229984 was significantly associated with Desire to cut drinking (p = 6.01E-14) and Tolerance (p=8.06E-9). An additional GWS region on chromosome 2 (rs7597960, p= 1.22E-8) was noted for Time spent drinking. The regions on chromosome 2, 4 and 8 survived the more stringent correction for the seven criteria (p≤1.67E-8) while the chromosome 15 variant was GWS but did not survive the additional correction for multiple testing of individual criteria (i.e., p= 1.77E-8). Conditional analyses demonstrated that there was only one association signal in the chromosome 15 region; however, the possibility of a second independent signal in the chromosome 8 region could not be ruled out (p<0.001) (Supplemental Figures 5D and 5E). Conditional analyses also suggested independent associations in the chromosome 2 region (Supplemental Figures 5B).
