Having confirmed similar pattern and time course of differentiation between BPD and control iPSCs, analysis of DEGs between BPD and controls at each time point was performed. Interestingly, there were no significant differences between BPD and controls at either NP or E stages of differentiation. However, L neurons from BPD cases compared to controls showed differential expressions for 697 probes, which corresponded to 324 genes. Out of the 324 genes, 292 genes showed upregulation in BPDs compared to their sibling controls while only 32 genes were downregulated. The complete list of DEGs between BPD and control L neurons is provided in Table 1.
