hCOs Recapitulate Human Dorsal Cortical Organization(A) Immunostaining for SOX2 and N-Cadherin in hCO section (40 day old). Arrows show potential oRGs outside of VZ-like area. Scale bar, 50 μm.(B and C) Immunostaining for SOX2, PAX6, Tuj1, and NeuN in hCO sections (40 day old). Arrows show potential oRGs outside of VZ-like areas. Scale bar, 50 μm.(D) GFAP staining in hCO section (40 day old). Arrow head: glial fibers; white arrow: vertically located RG cell; yellow arrow: horizontally located RG cell. Scale bar, 20 μm.(E) Quantification of position angle of RGs located at the lumen surface. Mean ± SD are plotted (n=4 VZ-like area from 4 of 40 day old hCOs).(F) Immunostaining for phospho-histone H3 in hCOs section (4 week old). Scale bar, 50 μm.(G) Representative images of vertical, oblique, and horizontal cleavage of dividing RGs in VZ-like area of 4 week old hCOs section (left). Mean ± SD are plotted for each category (n=42 cells). Scale bar, 10 μm.(H-J) Immunostaining for SOX2, GFAP, PAX6, TBR2, and FAM107A in hCO section (56 to 64 day old). Scale bar, 50 μm.(K) Immunostaining for Reelin in hCO section (64 day old). Scale bar, 25 μm.(L) Separation of deep layer CTIP2+ neurons and upper layer SATB2+ neurons in hCOs section. Scale bar, 50 μm.(M and N) Immunostaining for GFAP, NeuN, and MAP2 in hCO sections (105 day old). Scale bar, 25 μm.
