Transcriptome and Chromatin Accessibility during hMGEOs and hCOs Development(A) Enrichment of tissue-specific genes. Enrichment and depletion are scaled by –log10(FDR) andshown in yellow and blue colors, respectively.(B) Differentially-expressed genes during organoid development. Representative genes and GOterms (FDR<0.05) are shown in right panel.(C) Expression profile of key genes related to MGE and cortical development.(D) GSEA of gene signatures for in vivo embryonic brain region. Enrichment of gene signatures (-log10(FDR) in hCO and hMGEO is shown in blue and green, respectively.(E) Relationship between gene expression change and chromatin architecture during organoiddevelopment. Genes are sorted by log2(ratio) and the presence of dOCRs is shown by colors.(F) GO enrichment of target genes of dOCRs. –log10(FDR) is colored in red.(G) ATAC-seq read distribution around TSS of in vivo MGE and cortex-specific gene signatures.See also Figure S2.
