We used mtCOJO105 to individually condition the TUD-EUR summary statistics on loci associated with other comorbid traits, including alcohol dependence, cannabis use disorder and opioid use disorder. This analysis allowed us to examine whether the genetic associations with TUD would be preserved when controlling for those covariate phenotypes. To test as many SNPs while preserving computational efficiency, we used a p value threshold of 5.00E-06, 5.00E-08, 5.00E-06, respectively, for alcohol dependence, cannabis use disorder, and opioid use disorder. We then computed genetic correlations using the TUD summary statistics adjusted for the covariates of interest.
