TP63 is annotated with 15 coding variants in GENCODE 7, and all but 4 (TA-alpha, deltaN, P63delta and TP63-013) are rejected as potential principal isoforms by the SPADE (Pfam domains) and Matador3D (3D structure) modules in APPRIS. P63delta and TP63-013 are generated from TA-alpha and deltaN, respectively, by a known GYNGYN splicing event (45) that results in a swap of five amino acids ‘GTKRP’ for a single alanine in a non-critical region of the protein (Figure 3A). CORSAIR (vertebrate sequence database information) separates these four variants based on alignments with isoforms from other species (Figure 3B). It turns out that there is more ortholog evidence for deltaN and its GYNGYN variant TP63-013 than there is for TA-alpha and P63delta (the deltaN splice event is conserved as far back as chicken and Danio). There is also more CAGE data support for the deltaN/TP63-013 translation start site (A. Frankish, personal communication).
