For examining the acute impact of ethanol on synaptic transmission in human neurons, following baseline recordings, freshly made ethanol solution (40 mM) was perfused into the recording chamber for a duration of 5 min. For recordings under CIE conditions, coverslips with N40 or D40 neurons were moved into the chamber perfused with 40 mM ethanol during the entire electrophysiological recording period. For the control condition of CIE, ethanol was absent from sister N40 or D40 iN culture wells during the exposure paradigm and subsequent recordings.
