All data are presented as the mean ± standard error of the mean (SEM), unless otherwise noted. All experiments were repeated on at least three biological replicates, with each replicate consisting of an independent infection/differentiation. The effects of acute and chronic ethanol application were normalized to basal (baseline) activity in the absence of drug, i.e., drug effects are defined as the relative changes to baseline values (in the absence of drug). The effects of ethanol before and after the application in the same group of cells were compared using paired t tests (within-genotype differences). On normalized post-treatment data, one-way ANOVA tests were used to evaluate the ethanol effects between genotypes. To indicate statistical significance, the following symbols were used (*p < 0.05, **p < 0.01, ***p < 0.001) (NS = no statistical significance found). In the event that genotypic and within-genotype differences were found (#) are used to indicate significance for between-genotype effects and (*) for within-genotype effects.
